Journal: Scientific Reports

Article Title: Positive and negative cooperativity of TNF and Interferon-γ in regulating synovial fibroblast function and B cell survival in fibroblast/B cell co-cultures

doi: 10.1038/s41598-020-57772-7

Figure Lengend Snippet: Antibodies used for flow cytometry.

Article Snippet: Miltenyi , 130-102-643 , CD256 (APRIL) , APC.

Techniques: Cytometry, Membrane

Journal: PLoS ONE

Article Title: Generation and Characterisation of Cisplatin-Resistant Non-Small Cell Lung Cancer Cell Lines Displaying a Stem-Like Signature

doi: 10.1371/journal.pone.0054193

Figure Lengend Snippet: Antibody staining of PT and CisR cell lines for CD133 cell surface expression was carried out by flow cytometry using a CD133/1 (AC133) phycoerythrin (PE)-labelled antibody and IgG1 isotype control antibody. The percentage CD133+ cells were plotted for all cell lines (A). Differential expression of the CSC marker CD44 was examined using an anti-human CD44 FITC-conjugated antibody and corresponding IgG2b isotype control antibody. Expression levels of CD44 were determined for all cell lines and plotted as a percentage of the tumour cell population expressing CD44 (B). Data are expressed as Mean ± SEM from three independent experiments (n = 3) Data are expressed as Mean ± SEM from three independent experiments (n = 3) ( # p<0.01, * p<0.001).

Article Snippet: Cells (1×10 6 ) were incubated with either CD133/1 (AC133) phycoerythrin (PE)-labelled antibody or isotype control antibody (IgG1) (Miltenyi Biotec GmbH), or anti-human CD44 FITC-conjugated antibody and corresponding isotype control (IgG2b) (ImmunoTools GmbH, Germany) for 30 min in the dark at 4°C.

Techniques: Staining, Expressing, Flow Cytometry, Control, Quantitative Proteomics, Marker